GTP with a thieno[3,4-d]pyrimidine core (Thieno-GTP) is a fluorescent nucleotide that is readily accepted by T7 polymerase as a faithful GTP surrogate. This highly isomorphic nucleoside triphosphate is able to initiate transcription and incorporate during elongation. Transcripts containing a 5' pppthG are successfully dephosphorylated with alkaline phosphatase and subsequently phosphorylated with T4 polynucleotide kinase, illustrating that other terminus-modifying enzymes tolerate this modification as well.
Importantly, emissive messenger RNAs, substituted with Thieno-G are recognized by the ribosome and are capable of fluorescently reporting discrete steps in real time during translation. Additionally, Thieno-GTP can be utilized to visualize mRNA localization and assess mRNA delivery independent of translation.
| Catalog No | N-1088 |
|---|---|
| Purity | ≥95% by AX-HPLC |
| Extinction Coefficient | 4,500 Lmol-1cm-1 at 316 nm |
| Molecular Formula | C11H16N3O14P3S (free acid) |
| Molecular Weight | 539.20 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | Thieno-GTP, thGTP, 2-Aminothieno[3,4-d]pyrimidine-ribonucleoside-5'-Triphosphate |
| Application | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Guanosine |
| Sugar Type(s) | RNA |
| Nucleotide Category | Base Modified RNA |
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