CleanCap® Reagent GG (3' OMe) - (N-7413)

CleanCap®Reagent AG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG

This product is now available upon request as a custom synthesis. It is not maintained as a stocked item.


CleanCap Reagent GG (3ʹOMe) is designed for the cotranscriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).

CleanCap GG (3' OMe) includes the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped using ARCA, whereas CleanCap GG (N-7133) has a naturally occurring 5' N7-Methylguanosine structure.

CleanCap can be used in conjunction with TriLink's catalog of modified NTPs.

Using the recommended conditions, transcription with CleanCap GG (3’OMe) results in 70-90% capped material, generating a Cap 1 structure and gives crude yields of ~1.5 mg per mL of transcription. Final capping is dependent upon the DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.

Product Details

Catalog No N-7413
Purity ≥95% by AX-HPLC
Extinction Coefficient 30,539 Lmol-1cm-1 at 255 nm
Molecular Formula C33H45N15O24P4 (free acid)
Molecular Weight 1159.60 g/mole (free acid)
Salt Form NH4+/TEA+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) CleanCap®Reagent AG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG
Application CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases
Backbone 5'-5'-Triphosphate
Base Analog(s) Adenosine, Guanosine
Sugar Type(s) RNA
Nucleotide Category Cap analogs

Certificate of Analysis

Intellectual Property

CleanCap For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at Patent or patent pending,see

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.