Biotin-16-Aminoallyl-2'-deoxycytidine-5'-Triphosphate can be used for enzymatic incorporation of biotin into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and 3' labeling with terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this dNTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.
Product Details
Catalog No
N-5002
Purity
≥90% by AX-HPLC
Extinction Coefficient
5,040 Lmol-1cm-1 at 290 nm
Molecular Formula
C32H53N8O17P3S (free acid)
Molecular Weight
946.70 g/mole (free acid)
Salt Form
Li+
Concentration
5 mM
Buffer
H2O
Recommended Storage
-20°C or below
Other Name(s)
Biotin-16-dCTP, Biotin-16-AA-dCTP
Application
Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
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