Reporter genes are model systems used by researchers to assess various characteristics, such as delivery of an mRNA to a cell and percentage of cells transfected. They usually have an easily measured property such as fluorescence, bioluminescence, or a colorimetric change, and can also be used to monitor the effect of an intervention in an mRNA, such as inclusion of modified bases.
TriLink offers a range of convenient reporter mRNAs for your cell culture and in vivo experiments.
For fluorescent reporters, choose between enhanced green fluorescent protein (EGFP) and mCherry. EGFP has an excitation peak of 488 nm and an emission peak at 509 nm, while mCherry has a peak absorption at 587 nm and emission at 610 nm. Contact us for your additional GFP mRNA transfection needs. TriLink also offers cyanine 5 labeled Fluc and EGFP mRNAs for direct fluorescent detection.
For bioluminescent imaging, we offer reporter genes luciferase, firefly luciferase (FLuc) and Renilla luciferase mRNAs. In the presence of luciferin or coelenterazine, respectively, the bioluminescent proteins expressed from these mRNAs release visible light. Bioluminescent reporters are useful for time-course studies in cells and in vivo. For example, β-galactosides (β-gal) mRNA can be used for colorimetric detection of transfected cells, and it converts the substrate 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) to a compound with an easily detectable blue color.