5-Hydroxymethyl-2’-deoxycytidine-5’-Triphosphate - (N-2060)

5-hme-dCTP, 5-hmdCTP, 5-Hydroxymethyl-dCTP

This product is now available upon request as a custom synthesis. It is not maintained as a stocked item.

Description

Methylated deoxycytidine analogs are well studied epigenetic modifications. 5-Hydroxymethyl-2'-deoxycytidine has recently been discovered in mammalian genomic DNA, but is substantially depleted in many types of human cancers. A strong inverse relationship has been demonstrated between the proliferation rate of cells and the global level of 5-hydroxymethyl-2'-deoxycytidine in both porcine and rat tissues, but no such relationship was observed for 5-formyl-2'-deoxycytidine and 5-carboxyl-2'-deoxycytidine.

Product Details

Catalog No N-2060
Purity ≥90% by AX-HPLC
Extinction Coefficient 7,700 Lmol-1cm-1 at 275 nm
Molecular Formula C10H18N3O14P3 (free acid)
Molecular Weight 497.10 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) 5-hme-dCTP, 5-hmdCTP, 5-Hydroxymethyl-dCTP
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Cytidine
Sugar Type(s) DNA
Nucleotide Category Base Modified DNA

Supporting Documents



Certificate of Analysis


Intellectual Property

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References

Wu, Susan C.; Zhang, Yi . Active DNA demethylation: many roads lead to Rome.

Münzel, Martin; Globisch, Daniel; Carell, Thomas . 5-Hydroxymethylcytosine, the sixth base of the genome.

McKernan, Kevin J.; Spangler, Jessica; Zhang, Lei; Tadigotla, Vasisht; McLaughlin, Stephen; Warner, Jason; Zare, Amir; Boles, Richard G. . Expanded genetic codes in next generation sequencing enable decontamination and mitochondrial enrichment.

Li, Zheng; Gu, Tian-Peng; Weber, Alain R.; Shen, Jia-Zhen; Li, Bin-Zhong; Xie, Zhi-Guo; Yin, Ruichuan; Guo, Fan; Liu, Xiaomeng; Tang, Fuchou; Wang, Hailin; Schär, Primo; Xu, Guo-Liang . Gadd45a promotes DNA demethylation through TDG.

Jiang, Wenjing; Wu, Linni; Duan, Junling; Yin, Huanshun; Ai, Shiyun . Ultrasensitive electrochemiluminescence immunosensor for 5-hydroxymethylcytosine detection based on Fe(3)O(4)@SiO(2) nanoparticles and PAMAM dendrimers.

Li, F;Wang, S;Yin, H;Chen, Y;Zhou, Y;Huang, J;Ai, S; . Photoelectrochemical Biosensor for DNA Formylation Detection in Genomic DNA of Maize Seedlings Based on Black Tio2-Enhanced Photoactivity of MoS2/WS2 Heterojunction

Zhou, Y;Yin, H;Sui, C;Wang, Y;Ai, S; . Photoelectrochemical detection of 5-hydroxymethylcytosine in genomic DNA based on M. HhaI methyltransferase catalytic covalent bonding

Wang, Q;Yin, H;Ding, J;Zhou, Y;Ai, S; . WS2/Bi/BiOBr Nanostructures for Photoelectrochemical Sensing of 5-Formyluracil-2?-deoxyuridine-5?-triphosphate through Hemin/G-Quadruplex Double Signal Amplification

Wang, Q;Yin, H;Zhou, Y;Cao, L;Yu, Z;Xu, Y;Ai, S; . Photoelectrochemical biosensor for 5?formylcytosine based on WS 2 /Bi/ Bi 2 O 2 CO 3 nanocomposite and rolling circle amplification

Ding, J;Gao, L;Cao, L;Zhou, Y;Wang, H;Duan, J;Yin, H;Sun, J;Ai, S; . Photoelectrochemical immunosensor for DNA hydroxymethylation based on PTCA-sensitized perovskite Bi4TaO8Cl



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