Contains 1.0 µmole each of 4 nucleotides:
Chain terminators are sugar modified nucleoside triphosphates, where the 2' and 3' hydroxyl groups are absent. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddNTPs are used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
| Catalog No | K-1004 |
|---|---|
| Components | 2',3'-Dideoxyadenosine-5'-Triphosphate, 2',3'-Dideoxyguanosine-5'-Triphosphate, 2',3'-Dideoxythymidine-5'-Triphosphate, 2',3'-Dideoxycytidine-5'-Triphosphate |
| Recommended Storage | -20°C or below |
| Application | Aptamers, PCR & RT-PCR |
| Backbone | Phosphodiester |
| Base Analog(s) | Adenosine, Cytidine, Guanosine, Thymidine |
| Sugar Type(s) | 2'-3'-Dideoxy |
| Other Name(s) | ddNTP Set, DNA Chain Terminator Set |
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
