Contains 1.0 µmole each of 4 nucleotides:
Chain terminators are sugar modified nucleoside triphosphates, where the 2' and 3' hydroxyl groups are absent. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddNTPs are used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
|Components||2',3'-Dideoxyadenosine-5'-Triphosphate, 2',3'-Dideoxyguanosine-5'-Triphosphate, 2',3'-Dideoxythymidine-5'-Triphosphate, 2',3'-Dideoxycytidine-5'-Triphosphate|
|Recommended Storage||-20°C or below|
|Application||Aptamers, PCR & RT-PCR|
|Base Analog(s)||Adenosine, Cytidine, Guanosine, Thymidine|
|Other Name(s)||ddNTP Set, DNA Chain Terminator Set|
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Mizrahi, Rena A.; Schirle, Nicole T.; Beal, Peter A. . Potent and Selective Inhibition of A-to-I RNA Editing with 2Ã¢â‚¬Â²-O-Methyl/Locked Nucleic Acid-Containing Antisense Oligoribonucleotides