2',3'-Dideoxyadenosine-5'-Triphosphate - (N-4001)


This product is now available upon request as a custom synthesis. It is not maintained as a stocked item.


2',3'-Dideoxyadenosine-5'-Triphosphate (ddATP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is widely useful in antiviral research and in a variety of biotechnology applications. ddATP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. "I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results." Michal Legiewicz Research Scientist Yale University

Product Details

Catalog No N-4001
Purity ≥95% by AX-HPLC
Extinction Coefficient 15,400 Lmol-1cm-1 at 258 nm
Molecular Formula C10H16N5O11P3 (free acid)
Molecular Weight 475.10 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) ddATP
Application Aptamers
Backbone 5'-Triphosphate
Base Analog(s) Adenosine
Nucleotide Category Sugar Modified

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Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

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